Response of STAT5, STAT3, and ERK1/2 to CEP-701–mediated inhibition of FLT3 in primary AML blasts.

Whole cell extract (50 μg/sample) from the experiment shown in Figure6 was available for 5 of the 8 patient samples (numbering is the same as in Figure 6). Samples were electrophoresed and transferred to membrane, then the membrane was probed, stripped, and reprobed serially with the designated antibodies (A) upper, phosphorylated STAT5; lower, STAT5. (B) Upper, phosphorylated STAT3; lower, STAT3. (C) Upper, phosphorylated ERK1/2; lower- ERK1/2. Those samples found by RT-PCR to harbor a FLT3/ITD mutation are denoted with an asterisk.