The induction of cell-surface activation molecules in T-helper cells upon nonspecific stimulation is reduced in the post–T-cell depletion period.

PBMCs were stimulated with immobilized anti-CD3 as described. At the indicated time points, T-helper cells of control samples and patient samples were identified by staining with anti-CD4 and gated; the density of expression of CD69 and of CD25 and of HLA-DR in unstimulated cultures (thin line) and stimulated cultures (thick line) was estimated by the mpc in histogram plots. Patient samples were obtained from a 12-year-old girl with Ewing sarcoma (patient 10) 1 month after completion of conventional high-dose chemotherapy.