Fig. 2.
Fig. 2. Complete NF-κB inactivation induces Bcl-xLdown-regulation in quiescent human blood T cells. / (A) Blood T cells were isolated from buffy coats by negative magnetic selection and cultured for 6 hours and 24 hours in the presence or absence of 1.5 μM mGTX, 1.5 μM GTX, or 48 μM PGA1. Whole-cell extracts were then prepared and analyzed by immunoblotting for Bax, Bcl-2, Bfl1, and Bcl-xL/S expression. (B) RNA was prepared from blood T cells cultured for 6 hours and 18 hours in the presence or absence of 1.5 μM mGTX or 1.5 μM GTX and analyzed by RT-PCR for expression of bcl-xL. As a control for quantification, glyceraldehyde phosphate dehydrogenase (GAPDH) was also amplified. These results are representative of at least 3 comparable experiments.

Complete NF-κB inactivation induces Bcl-xLdown-regulation in quiescent human blood T cells.

(A) Blood T cells were isolated from buffy coats by negative magnetic selection and cultured for 6 hours and 24 hours in the presence or absence of 1.5 μM mGTX, 1.5 μM GTX, or 48 μM PGA1. Whole-cell extracts were then prepared and analyzed by immunoblotting for Bax, Bcl-2, Bfl1, and Bcl-xL/S expression. (B) RNA was prepared from blood T cells cultured for 6 hours and 18 hours in the presence or absence of 1.5 μM mGTX or 1.5 μM GTX and analyzed by RT-PCR for expression of bcl-xL. As a control for quantification, glyceraldehyde phosphate dehydrogenase (GAPDH) was also amplified. These results are representative of at least 3 comparable experiments.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal