Fig. 6.
Fig. 6. Antisense knockdown ofbcl-xL induces human blood T cell apoptosis. / Blood T cells were isolated from buffy coats by negative magnetic selection, cultured, and treated for 24 hours with DMRIE-C alone (24 μL per 2 × 106 cells) or DMRIE-C combined withbcl-x AS or SC ODNs (8 μg per 2 × 106cells). (A) Whole-cell extracts were prepared and analyzed by immunoblotting for Bcl-xL and Bcl-2. (B) Alternatively, apoptosis assays using dual-color annexin-V–FITC/PI staining and flow cytometry analyses were performed on treated T cells. The percentage of single- or double-positive cells in the individual quadrants is shown. These results are similar to at least 3 comparable experiments.

Antisense knockdown ofbcl-xL induces human blood T cell apoptosis.

Blood T cells were isolated from buffy coats by negative magnetic selection, cultured, and treated for 24 hours with DMRIE-C alone (24 μL per 2 × 106 cells) or DMRIE-C combined withbcl-x AS or SC ODNs (8 μg per 2 × 106cells). (A) Whole-cell extracts were prepared and analyzed by immunoblotting for Bcl-xL and Bcl-2. (B) Alternatively, apoptosis assays using dual-color annexin-V–FITC/PI staining and flow cytometry analyses were performed on treated T cells. The percentage of single- or double-positive cells in the individual quadrants is shown. These results are similar to at least 3 comparable experiments.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal