Human FIX expression in C57Bl/6 mice after injection of the recombinant first-generation adenoviral vectors.

Mice were injected with first-generation adenoviral vectors shown in Figure 1A-D. At different time points, blood samples were collected and analyzed by ELISA for hFIX expression. For the first-generation adenovirus shown in Figure 1A, an MOI of 20 was injected (♦). For the first-generation adenoviruses shown in Figure 1B (○ and ●), Figure1C (■ and ▪), and Figure 1D (▵ and ▴), an MOI of 2 (■/ ○/ ▵) and an MOI of 20 (●/▪/▴) was injected. Mean ± SD is shown (n = 3 per group).