Fig. 5.
![Fig. 5. DNA analysis of transduced adenoviral vector DNA in genomic liver DNA. / C57Bl/6 mice were injected with 2 × 109 transducing particles of either AdFTC/hFIX or fgAdhFIX. At both 5 days and 1 year after injection, genomic liver DNA was isolated and digested with HindIII, and a Southern blot was performed (n = 2 per group). Blots were hybridized with an α-32]dCTP–labeled cDNA hFIX probe, and the copy was determined by comparing the signal intensity of the resulting band with a standard curve that was obtained by digesting known amounts of the plasmid pBSh7hF9mgbpA containing the identical expression cassette withHindIII.19](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/11/10.1182_blood.v99.11.3923/6/h81122627005.jpeg?Expires=1735511528&Signature=oTkQJvqJLpCNqWtp9x0yWoC1deCmxThvYQ13l1bYsgUTLh05N7EPaylDFtubRSJHvRoLiGmgyfa0mStc1XVaivGYzbnbRhIT0bHQZn4DYqJRBedKrgKaHiDqwwvMdrBFMbVzwdDQ4aGeo1Yt0rMsAioA2Jmtew6Os24p4tY2NbRkpSrVk0xxBwVJKz8dOFUdGNew9rLAFF5RjkdCcqI8rA5OVh27VBEh2mN7FIe6VBo7ZCtTM4PTnyJTM45UjR9eIrwZrrS54CTpIR7UTl9ee5jQ48lz8DdYX0dcqdTWKzYJN8F-YXsoNY~xU3FOyowC5nDrSq5zwME0hNx5Hfm8FQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
DNA analysis of transduced adenoviral vector DNA in genomic liver DNA.
C57Bl/6 mice were injected with 2 × 109 transducing particles of either AdFTC/hFIX or fgAdhFIX. At both 5 days and 1 year after injection, genomic liver DNA was isolated and digested with HindIII, and a Southern blot was performed (n = 2 per group). Blots were hybridized with an α-32]dCTP–labeled cDNA hFIX probe, and the copy was determined by comparing the signal intensity of the resulting band with a standard curve that was obtained by digesting known amounts of the plasmid pBSh7hF9mgbpA containing the identical expression cassette withHindIII.19
