Fig. 3.
Fig. 3. CHO-131 staining of the transfectant Molt-4/FucT-VII but not of Jurkat/FucT-VII. / The transfected cell lines Molt-4/FucT-VII and Jurkat/FucT-VII were labeled with CHO-131 (15 μg/mL) or the anti-sLeX mAb HECA-452 (15 μg/mL), as indicated. Nonspecific antibody labeling was determined with the use of the appropriate isotype negative control mAbs (dashed line; mouse IgM control is shown). Cell staining levels were examined by flow cytometry, and 10 000 cells were examined per sample. Representative data from multiple repetitions are shown.

CHO-131 staining of the transfectant Molt-4/FucT-VII but not of Jurkat/FucT-VII.

The transfected cell lines Molt-4/FucT-VII and Jurkat/FucT-VII were labeled with CHO-131 (15 μg/mL) or the anti-sLeX mAb HECA-452 (15 μg/mL), as indicated. Nonspecific antibody labeling was determined with the use of the appropriate isotype negative control mAbs (dashed line; mouse IgM control is shown). Cell staining levels were examined by flow cytometry, and 10 000 cells were examined per sample. Representative data from multiple repetitions are shown.

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