Fig. 5.
Fig. 5. Distribution of the CHO-131 epitope on lymphocyte subsets. / (A) CHO-131 stains CD4+ effector/memory T cells. Human peripheral blood, gated on lymphocytes (10 000 cells), were dual analyzed for their expression of the CHO-131 epitope and CD3, CD4, or CD45RO, as indicated. (B) CHO-131 stains a subset of CLA+lymphocytes. Peripheral blood lymphocytes, gated on CD3+lymphocytes (10 000 cells), were dual analyzed for their expression of CLA (HECA-452) and C2-O-sLeX (CHO-131), as indicated. Nonspecific antibody labeling was determined with the use of the appropriate isotype negative control mAbs (data not shown). Cell-staining levels were examined by flow cytometry. Representative data from multiple repetitions are shown.

Distribution of the CHO-131 epitope on lymphocyte subsets.

(A) CHO-131 stains CD4+ effector/memory T cells. Human peripheral blood, gated on lymphocytes (10 000 cells), were dual analyzed for their expression of the CHO-131 epitope and CD3, CD4, or CD45RO, as indicated. (B) CHO-131 stains a subset of CLA+lymphocytes. Peripheral blood lymphocytes, gated on CD3+lymphocytes (10 000 cells), were dual analyzed for their expression of CLA (HECA-452) and C2-O-sLeX (CHO-131), as indicated. Nonspecific antibody labeling was determined with the use of the appropriate isotype negative control mAbs (data not shown). Cell-staining levels were examined by flow cytometry. Representative data from multiple repetitions are shown.

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