Flow cytometric analysis of the maternal serum Duv binding with the Duv^a+ allelic form of the GP IIb-IIIa complex.

Cos cells expressing the Duv^a− or the Duv^a+form of the GP IIb-IIIa complex were incubated with a serum containing an anti–HPA-1a antibody (A) and with the maternal serum Duv (B) or an AB control serum (A and B, dotted line). Incubated cells were rinsed prior to incubation with an antihuman IgG conjugated to phycoerythrin. Washed cells were analyzed by a FACSCalibur cytometer. The maternal serum Duv reacts specifically with the cells expressing the Duv^a+ allelic form of the GP IIb-IIIa complex, whereas positive reaction was observed for both types of cells with anti–HPA-1a.