Analysis of the binding of soluble fibrinogen and of the Moab PAC-1.

CHO cells stably transfected and expressing the Duv^a− or Duv^a+ allelic forms of the GP IIb-IIIa were treated by 10 mM DTT (bold line) or buffer (dotted line) for 20 minutes prior to being incubated with 200 μg/mL fibrinogen-A488 (A) or with 1 μg/mL of the Moab PAC-1 (B). Washed cells were directly analyzed for the fibrinogen binding by flow cytometry or incubated with an IgG anti-IgM conjugated to phycoerythrin to detect PAC-1 binding and analyzed. The Duv^a+ form of GP IIIa did not impair fibrinogen or PAC-1 binding to the complex.