Adhesion of transfected CHO cells onto immobilized fibrinogen.

Transfected cells expressing the Duv^a+ or the Duv^a− forms of the GP IIb-IIIa complex were incubated for 2 hours at 37°C in microtiter plates coated with different concentration of fibrinogen, BSA, or 20 μg/mL of the anti-GP IIb-IIIa Moab AP-2. Nonadherent cells were removed by washing, and adherent cells were fixed and stained in a crystal-violet solution for 30 minutes at room temperature. After washing and cell lysis in SDS, absorbance was measured at 560 nm. Cell adhesion was normalized to cell adhesion on AP-2 (fibrinogen/AP-2 ratio). Both cell types adhere onto fibrinogen in a dose-dependent manner. Results represent the mean ± SD of 3 experiments.