Phosphorylation of pp125^FAK.

CHO cells expressing the Duv^a+ or the Duv^a−forms of the GP IIb-IIIa complex were layered onto immobilized fibrinogen (Adh.) or maintained in suspension (Susp.) for 2 hours at 37°C before the cells were lysed and the pp125^FAKimmunoprecipitated. (A) Blots were performed to detect phosphotyrosine (blots P-tyr) and the pp125^FAK (blots FAK) by using the PY-20 and an anti-pp125^FAK Moabs, respectively. Detection was performed by using an antimouse IgG conjugated to the peroxidase associated to a chemiluminescent detection system. (B) The histogram represents the mean phosphorylation percentage for each cell line, calculated from the band intensities measured by densitometric analysis (see “Materials and methods”). Results represent the mean ± SD of 3 experiments.