American Society of Hematology

Fig. 3.

$Fig. 3. Tyrosine phosphorylation status of TM4SF-associated and total c-kit fractions in the absence or the presence of SLF. / MO7e cells were stimulated with or without 50 ng/mL SLF at 37°C for 5 minutes. Cells were solubilized with lysis buffer containing 1% CHAPS. Cell lysates were immunoprecipitated with indicated antibodies. After being separated by SDS-PAGE, proteins were immunoblotted with antiphosphotyrosine antibody (upper panel). The same membrane was reprobed with anti–c-kit polyclonal antibody (lower panel). The relative tyrosine phosphorylation levels normalized by c-kit protein amounts shown in lower panel were indicated at the bottom. These are the representative results from 3 separate experiments.$

Tyrosine phosphorylation status of TM4SF-associated and total c-kit fractions in the absence or the presence of SLF.

MO7e cells were stimulated with or without 50 ng/mL SLF at 37°C for 5 minutes. Cells were solubilized with lysis buffer containing 1% CHAPS. Cell lysates were immunoprecipitated with indicated antibodies. After being separated by SDS-PAGE, proteins were immunoblotted with antiphosphotyrosine antibody (upper panel). The same membrane was reprobed with anti–c-kit polyclonal antibody (lower panel). The relative tyrosine phosphorylation levels normalized by c-kit protein amounts shown in lower panel were indicated at the bottom. These are the representative results from 3 separate experiments.

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