Fig. 7.
Degradation patterns of c-kit protein after stimulation of MO7e cells with SLF.
Cellular proteins were labeled with35S-methionine-cysteine. Thereafter, the cells were treated with 50 ng/mL SLF at 37°C for indicated time periods. The cells were solubilized with lysis buffer containing 1% CHAPS and processed for immunoprecipitation with anti-CD63 mAb or anti–c-kit polyclonal antibody. CD63 immunoprecipitates were eluted with 1% NP-40 lysis buffer and supernatants were immunoprecipitated with anti–c-kit polyclonal antibody. Immunoprecipitated proteins were separated by SDS-PAGE and detected by autoradiography. These are the representative results from 2 separate experiments.
