PSGL-1 and PSGL-1Δcyto transfectants are appropriately posttranslationally modified and can recognize soluble P-selectin.

(A) WCLs were generated from PSGL-1 (lane 1) or PSGL-1Δcyto (lane 2) transfectants and immunoprecipitated with the anti-PSGL-1 mAb KPL1 coupled to Affigel followed by Western blotting with HECA-452. Arrows on right indicate dimeric (top arrow) and monomeric (lower arrow) forms of PSGL-1. Both full-length and truncated PSGL-1 were recognized by HECA-452. (B) PSGL-1 and PSGL-1Δcyto transfectants were incubated with P-RIg (filled histograms) or second stage only (open histograms) and analyzed by flow cytometry as described in “Materials and methods.” Both transfectants were capable of binding soluble P-selectin.