Disruption of the actin cytoskeleton in PSGL-1 transfectants blocks rolling on P-selectin.

(A) PSGL-1 transfectants were treated with either 10, 30, or 100 μM cytochalasin B (open squares), or 5, 50, or 100 μM latrunculin B (filled squares) and analyzed for rolling on CHO/P. To facilitate comparison between experiments, rolling events were normalized to the control group, which consisted of cells treated with vehicle alone (dimethyl sulfoxide). One of 3 similar experiments. (B) Expression of PSGL-1 on cells treated with 50 μM latrunculin B (open histogram) and untreated (filled histogram) PSGL-1 transfectants. Similar results were obtained when cells were treated with 100 μM cytochalasin B (data not shown).