Karyotype analysis in CD4⁺, CD56⁺ DC2 acute leukemia patient UPN3.

Representative R-banded (A) and M-FISH-Metasystems probe (B) karyotypes. Arrows indicate the presence of chromosomal abnormalities; large arrows identify recurrent anomalies in DC2 acute leukemia; small arrows identify other chromosomal changes. Modal number is 42. M-FISH allows to interpret add(1)(q41) as a der(1)t(1;8), add(2) as a dup(2), der(20) as a der(20)t(20;21), and add(21)(q22) as a der(21)(21p13 → 21q22::12::21q11→21qter). M-FISH also confirms the complete loss of chromosomes 9, 13, and 15 as well as partial 5q13q33 monosomy.