Fluorescently labeled MIP-1β colocalizes with cell membrane GM1.

CEM-R5 cells were stained simultaneously with CT-B–AF594 and biotinylated–MIP-1β followed by avidin-fluorescein (FITC). The MIP-1β binding is shown in green and the CT-B–AF594 staining (equivalent to Texas Red) is shown in red. Colocalization is indicated by a yellow and/or orange color in the overlay panel. Representative examples of 2 images are presented. Magnification, × 500.