The human SCL YAC completely rescues the

scl^−/− embryonic lethal phenotype.PCR genotype analysis of offspring derived from interbreeding YAC^+/−scl^+/− male and YAC^+/−scl^+/− female mice from line 2074. Lanes 1 and 2, YAC^+/−scl^+/−male and female parents; lanes 3 to 9, offspring with the following genotypes: 3, (YAC⁻scl^+/−); 4, (YAC⁺scl^+/+); 5, (YAC⁺scl^+/−); 6-9, (YAC⁺scl^−/−); +ve, positive control (YAC⁺scl^+/−); −ve, no DNA control.