Detection of human SCL protein in rescued YAC⁺

scl^−/− E14.5 fetal liver. Cell lysates were prepared from E14.5 fetal livers obtained from transgenic (line 2054) and nontransgenic mice. These were immunoprecipitated (IP) with rabbit antimouse SCL antisera or preimmune rabbit serum as indicated. The immunoprecipitates were subsequently Western blotted using an antimouse SCL antibody raised in sheep. Lysates from SCL-expressing cell lines (K562, human erythroleukemia; M1, mouse myeloid leukemia) and an SCL-nonexpressing cell line (J558L, mouse plasmacytoma) were included on the immunoblot as controls.