Analysis of hematopoietic progenitors and yolk sac angiogenesis in rescued YAC⁺

scl^−/− mice. Hematopoietic progenitor assays were performed using adult bone marrow (A,B) or yolk sac (C) from mice of the indicated genotype derived from line 2054. In each case, histograms represent the mean of 6 cultures from 2 mice. (A) Myeloid and erythroid (erythroid burst-forming unit [BFU-E]) colonies obtained from adult bone marrow from line 2054. Myeloid colonies were obtained by growth either in IL-3 and SCF or IL-3 and Tpo. (B) Myeloid colony types obtained by growth in IL-3 and SCF. (C) Yolk sac BFU-Es obtained from line 2133 or 2054. (D) Yolk sac vasculature from E9.5 embryos of the indicated genotype from line 2074. Note the large vitelline vessels present in both control and rescued yolk sacs.