Augmentation of GVHD is associated with reduction of GVL effects in B6 mice transplanted with CD4-depleted splenocytes from IFN-γ–deficient GKO BALB/c mice.

B6 mice were irradiated and transplanted with 5 × 10⁶ B6 TCD BMC (▪, syngeneic), or 5 × 10⁶ TCD BMC and 10 × 10⁶ CD4-depleted splenocytes from WT (○, WT BALB) or GKO (●, GKO BALB) BALB/c donors. Leukemic recipients were also injected with 500 EL4 cells along with the BMT inoculum. Data shown are survival (A) and survivors' body weights (B) of nonleukemic recipients, and survival of leukemic recipients receiving 500 EL4 cells (C).