Immunohistochemical localization of Cyr61 and CTGF in advanced atherosclerotic lesions of ApoE^−/− mice.

Frozen serial sections were taken from the heart at the aortic valve where prominent atherosclerotic lesions had formed. Sections were treated with H₂O₂, blocked with normal goat serum, and incubated with normal rabbit IgG (A,D), anti-Cyr61 (B,E), or anti-CTGF (C,F). Antibody localization was detected with a biotinylated secondary antibody, followed by the avidin-biotin-HRP labeling system using diaminobenzidine as the substrate. The sections were counterstained with hematoxylin and eosin. Lesions located at the aortic valve (A-C) and coronary artery (D-F) are shown.