Fig. 2.
Fig. 2. Specificity of immunohistochemical staining of Cyr61 in mouse atherosclerotic lesions. / Frozen serial sections, taken from the hearts of ApoE−/−mice, were incubated with rabbit IgG (A) or anti-Cyr61367–381 (B,C). To demonstrate staining specificity in panel C, anti-Cyr61367–381 was preabsorbed with 5 μg/mL recombinant Cyr61 for 16 hours at 4°C prior to its application to the sections. Immunohistochemical staining was performed as described in the legend of Figure 1.

Specificity of immunohistochemical staining of Cyr61 in mouse atherosclerotic lesions.

Frozen serial sections, taken from the hearts of ApoE−/−mice, were incubated with rabbit IgG (A) or anti-Cyr61367–381 (B,C). To demonstrate staining specificity in panel C, anti-Cyr61367–381 was preabsorbed with 5 μg/mL recombinant Cyr61 for 16 hours at 4°C prior to its application to the sections. Immunohistochemical staining was performed as described in the legend of Figure 1.

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