Adhesion of THP-1 cells to Cyr61 and CTGF.
(A) Washed THP-1 cells were incubated with vehicle buffer (control), 20 μM ADP, or 20 nM PMA as indicated and added to microtiter wells (1 × 105 cells/well) coated with 10 μg/mL Cyr61, CTGF, or BSA. Cell adhesion proceeded for 20 minutes at 37°C. (B) THP-1 cells were stimulated with 20 μM ADP, added to wells coated with the indicated concentrations of Cyr61, and allowed to adhere for 20 minutes at 37°C. (C) ADP-stimulated THP-1 cells were added to wells coated with 15 μg/mL Cyr61 or BSA and incubated for the indicated times. Nonadherent cells were removed by washing. Adherent cells were fixed, stained with methylene blue, and A650 of the extracted dye was measured. Data presented are means of triplicate determinations and error bars represent SEs. Figures are representative of 3 experiments.
