Fig. 4.
Activation-dependent adhesion of peripheral blood monocytes to Cyr61.
Isolated monocytes were preincubated with or without 2 mM EDTA for 30 minutes, and added to microtiter wells (2-3 × 105cells/well) coated with 10 μg/mL Cyr61 in the presence of vehicle buffer (control), 20 μM ADP, or 1 μM fMLP. After incubation for 20 minutes at 37°C, nonadherent cells were washed and adherent cells were detected by the acid phosphatase assay. Data shown are means of triplicate determinations and error bars represent SEs. Figure is representative of 2 experiments.
