Inhibition of monocyte adhesion to Cyr61.

Isolated monocytes were preincubated with inhibitors for 30 minutes at 37°C, then activated with 20 μM ADP, and allowed to adhere for 20 minutes at 37°C to microtiter wells coated with 10 μg/mL Cyr61, 10 μg/mL vitronectin, or 15 μg/mL fibrinogen as indicated. Cell adhesion was measured by the acid phosphatase assay. (A) Monocytes were pretreated with vehicle buffer (control), 200 nM mouse IgG (mIgG), 200 nM LM609 (anti-α_vβ₃), or 1 μM echistatin. Cell adhesion to Cyr61 (open bars) or vitronectin (closed bars) was performed. (B) Monocytes were pretreated with vehicle buffer (control), 200 nM mouse IgG (mIgG), 6S6 (anti-β₁), YFC118.3 (anti-β₂), or 2LPM19c (anti-α_M) for 30 minutes at 37°C. Cell adhesion to Cyr61 was performed. (C) Monocytes, pretreated with vehicle buffer (control), 200 nM mouse IgG, 2LPM19c (anti-α_M), or CBR-p150/4G1 (anti-α_X) for 30 minutes at 37°C, were allowed to adhere to Cyr61 (open bars) or fibrinogen (closed bars). Data shown are means of triplicate determinations and error bars represent SEs.