Fig. 8.
Fig. 8. Binding of the αMI domain to Cyr61 and CTGF. / Microtiter wells were coated with the indicated concentrations of Cyr61 (A) or CTGF (B), and blocked with 0.15% PVA. GST-αMI fusion protein or GST (1 μM each) was added and allowed to bind for 2 hours at 22°C. After washing, bound GST-αMI or GST was detected using a polyclonal anti-GST antibody followed by an HRP-conjugated secondary antibody. Bound antibodies were detected usingo-phenylenediamine dihydrochloride as the substrate and A490 was measured. (C) GST-αMI was preincubated with vehicle buffer (control), 500 nM YFC118.3 (anti-β2), or 2LPM19c (anti-αM) for 30 minutes at 37°C prior to addition to wells coated with 30 μg/mL Cyr61 (open bars) or CTGF (closed bars). Data shown are means of triplicate determinations and error bars represent SEs.

Binding of the αMI domain to Cyr61 and CTGF.

Microtiter wells were coated with the indicated concentrations of Cyr61 (A) or CTGF (B), and blocked with 0.15% PVA. GST-αMI fusion protein or GST (1 μM each) was added and allowed to bind for 2 hours at 22°C. After washing, bound GST-αMI or GST was detected using a polyclonal anti-GST antibody followed by an HRP-conjugated secondary antibody. Bound antibodies were detected usingo-phenylenediamine dihydrochloride as the substrate and A490 was measured. (C) GST-αMI was preincubated with vehicle buffer (control), 500 nM YFC118.3 (anti-β2), or 2LPM19c (anti-αM) for 30 minutes at 37°C prior to addition to wells coated with 30 μg/mL Cyr61 (open bars) or CTGF (closed bars). Data shown are means of triplicate determinations and error bars represent SEs.

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