RT-PCR analysis of the expression of caspase-8a, -8b, and -8L mRNA in normal human tissues and tumor cell lines.

(A) Complementary DNAs of selected human normal tissues (obtained from CLONTECH) were amplified by PCR with primers that detect caspase-8a, -8b, and -8L. (B) Total RNAs from human tumor cell lines were reverse transcribed and then amplified by PCR with the same primers used in panel A. The resulting PCR products were analyzed on 1.2% agarose gel and stained by ethidium bromide. Caspase-8a, -8b, and -8L fragments are indicated by arrows. As an internal control for RT-PCR, glyceraldehyde-3-phosphate dehydrogenase cDNA was amplified by PCR under the same experimental conditions and was used for normalization.