Expression of GPIbα on recombinant cell lines.

Transfected cell lines were incubated with the mAbs to GPIbα (A) AK3 and (B) AK2 and cell autofluorescence was detected using a mouse isotype control antibody. Bound mAbs were detected using a FITC-labeled antimouse IgG antibody, followed by flow cytometric analysis (n = 2-3). (C) The data for GPIbα expression are also presented as a ratio of bound AK2 to AK3 binding for the mutants normalized to wild-type expression.