Bisulfite sequencing of the CpG island associated with the

RFP2 gene in 3 B-CLL patients. DNA was bisulfite-converted, PCR-amplified, and cloned, and single clones were sequenced. (A) In the genomic sequence of the 5′ end of theRFP2 gene, the transcription start of RFP2 is shown as an arrow, CpG doublets are depicted as open circles and numbers are bp. (B) Sequences of clones are shown as lines and methylated cytosines as vertical lines.