American Society of Hematology

Fig. 4.

$Fig. 4. Hemin activates human neutrophil PKC. / Human neutrophils were incubated for 30 minutes in the presence of different concentrations of hemin, as indicated on the abscissa. After that, cells were ruptured as described in “Materials and methods,” and PKC activity was determined in the cytosolic fraction in the presence (+BIM) or absence (-BIM) of 10 nM BIM. Results are expressed as a percentage of the control activity, determined from cells treated with 0.01% DMSO. Data are presented as mean ± SD from 2 different experiments performed in triplicate.$

Hemin activates human neutrophil PKC.

Human neutrophils were incubated for 30 minutes in the presence of different concentrations of hemin, as indicated on the abscissa. After that, cells were ruptured as described in “Materials and methods,” and PKC activity was determined in the cytosolic fraction in the presence (+BIM) or absence (-BIM) of 10 nM BIM. Results are expressed as a percentage of the control activity, determined from cells treated with 0.01% DMSO. Data are presented as mean ± SD from 2 different experiments performed in triplicate.

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