![Fig. 5. The expression of IL-8 mRNA in human neutrophils following exposure to hemin. / Neutrophils (5 × 106 cells) were incubated for 30 minutes at 37°C with 3 μM hemin in the presence (H3 + HSA) or absence (H3) of 1% human serum albumin, or in the presence of 10 nM BIM (H3 + BIM). Control groups received only PBS (control) or albumin (HSA) as treatment. For positive IL-8 transcript production, cells were treated with 10 ng/mL LPS. After that, cells were washed 2 times with PBS and incubated for 4 hours in RPMI. Total cellular RNA was isolated, and RT-PCR was performed using IL-8 and GAPDH-specific probes in the presence of α[32P]-CTP. Data are presented as the ratio between the expression of IL-8 to GAPDH levels. For additional information, see “Materials and methods.” This representative experiment was repeated 3 times, showing similar results. Data are reported as cumulative data in the form of mean ± SD. *P < .01.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/11/10.1182_blood.v99.11.4160/6/h81122605005.jpeg?Expires=1735858043&Signature=07ScfTsfVWcdswXv~lInSl4UQWL8fY7QDPnAH8CGBVo74AxnH470Db2k2QhPqxtZLH8bkOZklr~QDaFLVYBvzu~DY6vfeo5Uj3jcR1wL7PrWWp~qb6keoWVMWfP8kQg-elZF1mi3fE1VALkhHuUyrJUxSlwW5L1zNYXjffgOtCltNNVaESVc3w9s3zAUjjgczl1XnhlZrZTyzVwcGswNiRhlO-XvyTJHi8c7u2i0JtS33WanIt~AxttGJAAqiKCcW6llaZAeQXp1hLH4icWOIH8pg0~6yIoloBDeGIfL-P~4nTVw4Tgk1~HraPT0VSAviuVDamQpGjzD74ktNn5RFQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The expression of IL-8 mRNA in human neutrophils following exposure to hemin.
Neutrophils (5 × 106 cells) were incubated for 30 minutes at 37°C with 3 μM hemin in the presence (H3 + HSA) or absence (H3) of 1% human serum albumin, or in the presence of 10 nM BIM (H3 + BIM). Control groups received only PBS (control) or albumin (HSA) as treatment. For positive IL-8 transcript production, cells were treated with 10 ng/mL LPS. After that, cells were washed 2 times with PBS and incubated for 4 hours in RPMI. Total cellular RNA was isolated, and RT-PCR was performed using IL-8 and GAPDH-specific probes in the presence of α[32P]-CTP. Data are presented as the ratio between the expression of IL-8 to GAPDH levels. For additional information, see “Materials and methods.” This representative experiment was repeated 3 times, showing similar results. Data are reported as cumulative data in the form of mean ± SD. *P < .01.
