Fig. 7.
Effect of exogenous PU.1 onp40phox promoter function.
HL-60 cells were transfected with the wild-type reporter vector pGL3-p40-106 or the analogous vector in which all 3 PU.1 sites were mutated (pGL3-p40-106-mtPU.1) and were cotransfected with either a wild-type PU.1 expression plasmid (pJ6-mPU.1), a dominant-negative PU.1 mutant plasmid (pJ6-NN), or the empty expression vector (pJ6). Reporter gene activity was assayed as before, and results were expressed as the mean (± SE) of 3 independent experiments.
