Fig. 1.
![Fig. 1. Activation of Akt, ERK1/2, and p90RSK in MO7e cells after stimulation with SDF-1α. / (A) MO7e cells were incubated with 100 ng/mL SDF-1α for the indicated time periods. (B) MO7e cells were preincubated in the presence of dimethyl sulfoxide (DM) vehicle control or LY 294002 (LY, 30 μM), wortmannin (W, 100 nM), PD 98059 (PD, 25 μM), or rapamycin (R [also called sirolimus], 10 nM) for 1 hour, and stimulated with 100 ng/mL SDF-1α for 5 minutes. Cell lysates were analyzed by Western blotting with phosphospecific antibodies to Akt (Ser473), ERK1/2 (Thr202/Tyr204), or p90RSK (Ser381). Amounts of p90RSK (A) or Akt (B) are shown on the bottom panel of each as a loading control. This experiment was performed 3 times with similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/12/10.1182_blood.v99.12.4307/4/h81222709001.jpeg?Expires=1735590850&Signature=g2Jr2YH4zXN3ppp3CNSGIY0onLptY8Ijrh8lfZj0kbzsk2RLF9zGrKUjEvmEpeKXKsJocTQv0OhtNV60ve5xFGTaW8zmXLf9YVv~cIAztvQv-~7jPZaq1REDiBNCJVloF8EgB7Yyx5ASoxWNCYQ2rXjMSyCVK6jy2cN7hadvliMZVH8g3QZ9~GAg1WO-lPM4~c0YLQ95pTlSq5NdQSe7W6lMs45jrKDJKBso1ve6n~Kg3TP48rYyg8EPFG811OnrjiNHXzFWt0mLJpnWjzVB5GfDMLAS9sUcf2P4YJRA3O4~ux41wc34wqcINJMUnX-ipgxhNdna81ww~o9orVGtnA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of Akt, ERK1/2, and p90RSK in MO7e cells after stimulation with SDF-1α.
(A) MO7e cells were incubated with 100 ng/mL SDF-1α for the indicated time periods. (B) MO7e cells were preincubated in the presence of dimethyl sulfoxide (DM) vehicle control or LY 294002 (LY, 30 μM), wortmannin (W, 100 nM), PD 98059 (PD, 25 μM), or rapamycin (R [also called sirolimus], 10 nM) for 1 hour, and stimulated with 100 ng/mL SDF-1α for 5 minutes. Cell lysates were analyzed by Western blotting with phosphospecific antibodies to Akt (Ser473), ERK1/2 (Thr202/Tyr204), or p90RSK (Ser381). Amounts of p90RSK (A) or Akt (B) are shown on the bottom panel of each as a loading control. This experiment was performed 3 times with similar results.
