Synergistic activation of Akt in MO7e cells by SDF-1α in combination with other cytokines.

Cell lysates were analyzed by Western blotting with phosphospecific antibodies to Akt (Ser473). The amount of total Akt is shown as a loading control in the bottom panels. (A) SDF-1α plus GM-CSF. (B) SDF-1α plus SLF. (C) SDF-1α plus TPO. (D) Columns represent relative band intensities of phospho-Akt ± SD from 3 experiments. *P < .05 (greater than additive).