Fig. 1.
Fig. 1. Molecular and cellular analyses of the BM-HPC lines. / (A) May-Grünwald Giemsa staining of the BM-HPCs. Magnification × 100. (B) Northern blot analysis showing Lhx2 expression in 3 independently generated BM-HPC lines compared with a cell line generated from ES cells (HPC line no. 7). (C) Southern blot analysis of genomic DNA derived from the 2 BM-HPC lines used in this study showing a single proviral integration site in both cell lines. Cell surface marker analyses showing the difference in expression of the stem cell marker Sca-1 on the BM-HPC lines generated in SF/IL-6 (BM-HPC no. 5) (D, top) and in SF alone (BM-HPC no. 9) (D, bottom). (E) Flow cytometry analyses of cell surface markers showing similar expression pattern on BM-HPC line nos. 5 and 9.

Molecular and cellular analyses of the BM-HPC lines.

(A) May-Grünwald Giemsa staining of the BM-HPCs. Magnification × 100. (B) Northern blot analysis showing Lhx2 expression in 3 independently generated BM-HPC lines compared with a cell line generated from ES cells (HPC line no. 7). (C) Southern blot analysis of genomic DNA derived from the 2 BM-HPC lines used in this study showing a single proviral integration site in both cell lines. Cell surface marker analyses showing the difference in expression of the stem cell marker Sca-1 on the BM-HPC lines generated in SF/IL-6 (BM-HPC no. 5) (D, top) and in SF alone (BM-HPC no. 9) (D, bottom). (E) Flow cytometry analyses of cell surface markers showing similar expression pattern on BM-HPC line nos. 5 and 9.

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