The BM-HPC lines are multipotent and generate functional cells upon transplantation into lethally irradiated mice.

(A) Kaplan-Meier plot of mice receiving a lethal dose (10 Gy) of radiation and transplanted with 3 × 10⁶ BM-HPCs (11 mice), 10⁶ (3 mice) BM-HPCs, or mock-transplanted controls (6 mice). Data for line nos. 5 and 9 are pooled. Solid line indicates control mice; broken line, mice receiving 10⁶cells; dotted line, mice receiving 3 × 10⁶cells. (B) Average donor contribution to circulating erythrocytes based on Gpi assays in surviving mice at different time points after transplantation. SE is included in each time point. Cell surface marker analyses for the presence of donor cells (CD45.2⁺ vertical axes) of different lineages (horizontal axes) in control B6-SJL mice (C) and in representative B6-SJL mice receiving either BM-HPC line no. 5 (D-J) or no. 9 (K-N) analyzed 6 to 8 weeks after transplantation. Percentage donor cells of different lineages is indicated in the upper right quadrant for the mice receiving transplants (D-N) and in the upper left quadrant in the control (C) (eg, no lineage marker is included). Organs analyzed were spleen (D-G,K-N) and lymph nodes (C,H-J).