American Society of Hematology

Fig. 4.

$Fig. 4. The BM-HPC lines engraft stem cell–deficient mice for an extended time. / Data for BM-HPC line nos. 5 and 9 are pooled because no difference in reconstitution ability could be observed. (A) Gpi analyses 1 month after transplantation of circulating erythrocytes in B6W mice receiving different doses of BM-HPCs. Displayed are the Gpi assays together with the calculated value for the fraction (in percent) of donor Gpi1a isoform for each individual mice receiving the indicated cell dose. (B) Percentage donor-derived erythrocytes in peripheral blood at different time points of 13 individual recipients receiving 3 × 106 BM-HPCs. Each line indicates an individual mouse. (C) Three examples of primary recipient animals (nos. 51, 64, 150) receiving 3 × 106 BM-HPCs showing no donor contribution to peripheral blood (PB) but significant donor contribution (≥ 50%) to nucleated cells in the BM. Bands corresponding to the respective Gpi isoforms are indicated.$

The BM-HPC lines engraft stem cell–deficient mice for an extended time.

Data for BM-HPC line nos. 5 and 9 are pooled because no difference in reconstitution ability could be observed. (A) Gpi analyses 1 month after transplantation of circulating erythrocytes in B6W mice receiving different doses of BM-HPCs. Displayed are the Gpi assays together with the calculated value for the fraction (in percent) of donor Gpi1^a isoform for each individual mice receiving the indicated cell dose. (B) Percentage donor-derived erythrocytes in peripheral blood at different time points of 13 individual recipients receiving 3 × 10⁶ BM-HPCs. Each line indicates an individual mouse. (C) Three examples of primary recipient animals (nos. 51, 64, 150) receiving 3 × 10⁶ BM-HPCs showing no donor contribution to peripheral blood (PB) but significant donor contribution (≥ 50%) to nucleated cells in the BM. Bands corresponding to the respective Gpi isoforms are indicated.

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