The BM-HPCs engraft after serial transplantation into secondary recipients.
(A) Fraction of donor-derived erythrocytes in peripheral blood at different time points of 22 individual secondary stem cell[–deficient B6W recipients. Fifty-five percent of the animals received 106 and 45% of the animals received 2 × 106BM cells from primary B6W recipients. (B) Fraction of donor-derived erythrocytes in peripheral blood at different time points of 13 individual secondary B6-SJL wild-type recipients. All mice received 2 × 106 BM cells from the primary B6W recipients. Each line indicates an individual mouse. (C) Three examples of secondary recipient animals (nos. 130, 165, 132) showing no donor contribution to peripheral blood (PB) but significant donor contribution (≥ 50%) to nucleated cells in the BM. Bands corresponding to the respective Gpi isoforms are indicated.
