The BM-HPCs engraft after serial transplantation into tertiary recipients.
(A) Fraction of donor-derived erythrocytes in peripheral blood at different time points of 7 individual tertiary stem cell–deficient B6W recipients. All mice received 2 × 106 BM cells from the secondary B6W recipient. The secondary recipient used was killed 6 months after transplantation. The secondary recipient received BM cells from a primary recipient killed 9 months after transplantation. (B) May-Grünwald Giemsa staining of nucleated cells from BM isolated from a tertiary recipient killed 4 months after transplantation. Original magnification B, × 40. (C) Gpi assay of the nucleated cells in the BM and of erythrocytes in peripheral blood (PB) of the same individual analyzed in panel B.
