Mice transplanted with BM-HPCs are engrafted with cells showing the same proviral integration site as the original BM-HPC line.

(A) Southern blot analysis of genomic DNA using Neo as a probe comparing the proviral integration site in the original BM-HPC lines (no. 5) with that of a spleen of a primary recipient (no. 103), a spleen of a secondary recipient (no. 179), and a spleen of a tertiary recipient (no. 225). All organs analyzed showed at least 50% donor contribution based on Gpi assays. Normal BM from the donor mouse strain (B6-cast) is used as negative control. (B) Similar to panel A, but analysis of the BM cells of the primary recipient analyzed in panel A (no. 103) and the BM and spleen of 2 additional primary recipients (nos. 105, 106) were also included.