Long-term expression of H-2K^b in peripheral blood leukocytes of mice reconstituted with VSV-Kb–transduced BM.

B10.AKM mice were preconditioned with anti-CD4 and anti-CD8 antibodies, lethally irradiated, and reconstituted with 4 × 10⁶VSV-Kb–transduced (squares) or mock-transduced (circles) syngeneic BM. At 4, 6, 8, 12, 16, 22, and 36 weeks after reconstitution, peripheral blood from these animals was examined for K^b expression by flow cytometry following cell surface staining. Shown are the percentages of K^b expressing cells. The values provided represent the mean and SD of a representative experiment containing a total of 11 VSV-Kb–transduced mice and 8 mock-transduced mice. Flow cytometry gates were set using cells from mice receiving mock-transduced BM. Dead cells were excluded from this analysis.