Mice reconstituted with VSV-Kb–transduced BM are tolerant to K^b.

B10.AKM mice reconstituted with VSV-Kb– or VSV-GFP–transduced BM received a B10.MBR and B10.BR skin graft 8 weeks after reconstitution and were immunized with 5 × 10⁶ irradiated B10.MBR splenocytes 28 weeks after reconstitution. Ten days after immunization, splenocytes from VSV-GFP and VSV-Kb mice were harvested and cultured for 5 days with irradiated B10.MBR stimulator cells. Splenocytes from control mice reconstituted with VSV-GFP–transduced BM were tested for their ability to kill ConA-treated B10.MBR splenocytes (squares) or Abelson transformed pre-B cells lines expressing K^b(TBA-Kb; circles). Splenocytes from mice reconstituted with VSV-Kb–transduced BM were tested for their ability to kill ConA-treated B10.MBR splenocytes (triangles) or TBA-Kb cells (diamonds). Results of CTL-mediated lysis after restimulation in vitro in the presence (A) or absence (B) of 20 IL-2 U/mL is shown from a representative experiment.