Cytokine-induced expression of PLSCR1.

Cultured bone marrow progenitors from 5 age-matched adult mice were incubated for 3 days in MethoCult 3234 supplemented with either 20 ng/mL SCF or 60 ng/mL G-CSF. Cells were harvested at indicated times, and cell lysates (6 μg total protein per lane) were analyzed by Western blotting for PLSCR1 antigen (see “Materials and methods”).