Detection of the ABL-BCR transcript by RT-PCR and Southern blotting.

Ethidium bromide–stained agarose gels (panel A) and Southern blots (panel B) from the same patients were run on different gels. Lanes 4-11 show nondeleted patients; lanes 16-23, deleted patients. Lanes 1 and 13 show HL6O (BCR-ABL–negative) cell line; lanes 2 and 14, Meg-1 cell line; lanes 3 and 15, LAMA cell line, lanes 12 and 24, water control. 1b-b3 indicatesABL1b-BCRb3 transcript; 1b-b4,ABL1b-BCRb4 transcript; PBGD, porphobilinogen deaminase transcript.