Fig. 2.
Fig. 2. GPVI cytoplasmic sequence and other calmodulin-binding sequences. / (A) GPVI cytoplasmic sequence, His269-Pro287, compared with calmodulin-binding sequences in GPIbβ (Arg149-Leu167),8a model calmodulin-binding control peptide CBCP,19 and L-selectin.20 Identical amino acids or conserved substitutions are boxed. (B) Helical wheel representation of the GPIbβ and GPVI cytoplasmic sequences. (C) Nondenaturing gel shift of calmodulin (0.3 nmol) in the presence of increasing concentrations of GPVI peptide, His269-Pro287. The shift is shown relative to that induced by a peptide based on the GPV calmodulin-binding sequence Lys529-Gly544.8 Upper and lower panels were electrophoresed in the presence of 1 mM Ca++ or 10 mM EGTA, respectively. Gels were stained with Coomassie blue.

GPVI cytoplasmic sequence and other calmodulin-binding sequences.

(A) GPVI cytoplasmic sequence, His269-Pro287, compared with calmodulin-binding sequences in GPIbβ (Arg149-Leu167),8a model calmodulin-binding control peptide CBCP,19 and L-selectin.20 Identical amino acids or conserved substitutions are boxed. (B) Helical wheel representation of the GPIbβ and GPVI cytoplasmic sequences. (C) Nondenaturing gel shift of calmodulin (0.3 nmol) in the presence of increasing concentrations of GPVI peptide, His269-Pro287. The shift is shown relative to that induced by a peptide based on the GPV calmodulin-binding sequence Lys529-Gly544.8 Upper and lower panels were electrophoresed in the presence of 1 mM Ca++ or 10 mM EGTA, respectively. Gels were stained with Coomassie blue.

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