American Society of Hematology

Fig. 2.

$Fig. 2. Patient plasma contains an antifactor V antibody. / All components of prothrombinase (factor V, factor Va, factor Xa) as well as APC were analyzed nonreduced on a 5% to 15% SDS-PAGE followed by transfer to nitrocellulose (approximately 1.5 μg of each protein was applied per lane). The isolated total immunoglobulin fraction from the patient's plasma (eluted from the protein G–Sepharose column) was used as a source of primary antibody (at a concentration of 500 μg/mL) and staining of the proteins was accomplished with an antihuman immunoglobulin coupled to horseradish peroxidase (1:5000 dilution) and chemiluminescence. The lane assignment is indicated on the top of the figure. Control experiments were performed using a purified immunoglobulin fraction from normal plasma.$

Patient plasma contains an antifactor V antibody.

All components of prothrombinase (factor V, factor Va, factor Xa) as well as APC were analyzed nonreduced on a 5% to 15% SDS-PAGE followed by transfer to nitrocellulose (approximately 1.5 μg of each protein was applied per lane). The isolated total immunoglobulin fraction from the patient's plasma (eluted from the protein G–Sepharose column) was used as a source of primary antibody (at a concentration of 500 μg/mL) and staining of the proteins was accomplished with an antihuman immunoglobulin coupled to horseradish peroxidase (1:5000 dilution) and chemiluminescence. The lane assignment is indicated on the top of the figure. Control experiments were performed using a purified immunoglobulin fraction from normal plasma.

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