Analysis of CD63 expression on the surface of purified crystalloid granules.

Highly purified crystalloid granules were immunostained and analyzed using flow cytometry technique. (A) Isotype control, (B) isotype control for saponin-permeabilized granules, (C) CD63 expression on the surface of nonpermeabilized crystalloid granules, and (D) CD63 expression in saponin-permeabilized granules. The MFI of permeabilized crystalloid granules was approximately 2-fold increased when compared with nonpermeabilized granules.