Fig. 5.
Inhibitory effect of rabbit antipeptide antibody IgG (residues 2009-2022) on FVIII bindings to anhydro-APC or VWF.
Serial dilutions of antipeptide IgG (○) or control rabbit IgG (●) were incubated with FVIII for 2 hours at 37°C prior to adding to immobilized anhydro-APC (A) or VWF (B). Bound FVIII was detected using peroxidase-conjugated MoAb JR8.